mouse monoclonal anti-dcp1a (3g4)  (Novus Biologicals)

Journal: Cell

Article Title: The Parkinson’s disease protein alpha-synuclein is a modulator of Processing-bodies and mRNA stability

doi: 10.1016/j.cell.2022.05.008

Figure Lengend Snippet: Key resources table

Article Snippet: Anti-DCP1A mouse monoclonal antibody (M06), clone 3G4 , Abnova , Cat# H00055802-M06, RRID:AB_530021.

Techniques: Western Blot, Virus, Recombinant, Protease Inhibitor, Lysis, Magnetic Beads, Membrane, Transfection, Expressing, Bicinchoninic Acid Protein Assay, Silver Staining, In Situ, Sample Prep, Luciferase, Reporter Assay, Multiplex sample analysis, Biomarker Discovery, Marker, Generated, Software, Mass Spectrometry, Imaging

Journal: iScience

Article Title: NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells

doi: 10.1016/j.isci.2021.102762

Figure Lengend Snippet: The Nanos2 TAG mouse allele is functional (A) Schematic representation of the NANOS2 protein and the TAG-NANOS2 fusion protein, with corresponding lengths of amino acid sequences (aa). (B) Number of litters per animal from Nanos2 CTL (Nanos2 +/+ ) and Nanos2 TAG ( Nanos2 TAG/TAG ) mice. Data are mean and s.d., n = 6 for both genotypes. n.s. indicates not significant, p value > 0.05 using two-tailed Student's t -test. (C) Testicular weight of Nanos2 CTL and Nanos2 TAG from six-months-old mice. Data are mean and s.d., n = 5 for both genotypes. Statistical evaluation as in panel b. (D) Representative testis cross-sections stained with hematoxylin and eosin (H&E) from six-months-old Nanos2 CTL and Nanos2 TAG mice. Scale bar, 100 μm. (E) Representative immunofluorescent images of Nanos2 TAG seminiferous tubules stained with anti-GFP (green) and anti-GFRα1 (red) antibodies. Representative examples of GFRα1 Pos , NANOS2 Pos cells (A s and A pr ) are highlighted (white boxes). Scale bar, 50 μm. (F) Enumeration of GFRα1 Pos NANOS2 Pos and GFRα1 Neg NANOS2 Pos testicular populations. The number of cells (N) present in each cluster is normalized to 1,000 Sertoli cells (y axis). Error bars represent SEM (standard error of mean). (G) Representative immunofluorescent images of Nanos2 TAG seminiferous tubules stained with anti-GFP (green) and anti-c-KIT (red) antibodies. Representative example of NANOS2 Pos , c-KIT Neg cell (A s ) is highlighted (white box). Scale bar, 50 μm. (H) Representative immunofluorescent image of an A s cell from Nanos2 TAG seminiferous tubules stained with with DAPI (blue), anti-GFP antibody (green) and anti-DCP1a (red). Scale bar, 5 μm. (I) Representative bright field images of Nanos2 CTL and Nanos2 TAG SSC lines cultured on mouse embryonic fibroblasts (MEF) feeders. (J) Western blot using anti-GFP and anti-Actin antibodies on Nanos2 CTL and Nanos2 TAG SSC lines lysates. (K) Flow cytometry analysis of Nanos2 CTL and Nanos2 TAG SSC lines. The GFP intensity of the Nanos2 TAG population is represented in a single-parameter histogram, with the overlay of the Nanos2 CTL control.

Article Snippet: Mouse monoclonal anti Dcp1a , Sigma-Aldrich , Cat#WH0055802M6; RRID: AB_1843673.

Techniques: Functional Assay, Two Tailed Test, Staining, Cell Culture, Western Blot, Flow Cytometry

Journal: iScience

Article Title: NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells

doi: 10.1016/j.isci.2021.102762

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti Dcp1a , Sigma-Aldrich , Cat#WH0055802M6; RRID: AB_1843673.

Techniques: Recombinant, Titration, Gel Extraction, Software

Journal: Cell

Article Title: Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s

doi: 10.1016/j.cell.2018.07.022

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Article Snippet: Mouse monoclonal anti-hDCP1A , Santa Cruz Biotechnology , sc100706; RRID: AB_2090408.

Techniques: Recombinant, Magnetic Beads, Reverse Transcription, Hybridization, SYBR Green Assay, Gene Expression, Ligation, Negative Control, Sequencing, Selection, Stable Transfection, Over Expression, Plasmid Preparation, Software, Flow Cytometry